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36B, C)

The RNA and primers were then annealed by incubating as follows: 90 C (1 min), 80 C (1 min), 70 C (1 min), 60 C (1 min), 50 C (1 min), and 40 C (6 min)

Understanding this pathway is not only key to elucidating the origin of SFN but also provides the essential toolkit for its sustainable enhancement through genetic biofortification

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In another aspect of this embodiment, the compound has the structure of formula (III): wherein the C 2-4 alkyl is selected from the group consisting of ethyl, isopropyl, n-propyl, butyl, and t-butyl, or an N-oxide, crystalline form, hydrate, or pharmaceutically acceptable salt thereof