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Description

In all qPCR experiments, 18s RNA expression was used to normalize gene expression within each tissue sample that was processed in triplicate

100 L of beads resuspension was incubated with 5 g of sucA-AMP (470 nM) and either no nucleotide, 1:1 molar ratio nucleotide (470 nM), 1:5 molar ratio nucleotide (2.35 M), or 1:25 molar ratio nucleotide (11.75 M)