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doi: 10.1073/pnas.2212476120 125 Azarcoya-BarreraJWollinBVeida-SilvaHMakarowskiAGorukSFieldCJet al

The PCR reaction mixtures were subjected to the following conditions: pre-denaturation at 95 for 30 s, followed by 40 cycles of 95 for 5 s, 60 for 30 s