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The dilution in the range of 10 -4 , 10 -5 , 10 -6 dilution were spread on nutrient agar, followed by incubation until the colonies are observed at 37 C

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Mani V, Chikkaveeraiah BV, Patel V, Gutkind JS, Rusling JF (2009) Ultrasensitive immunosensor for cancer biomarker proteins using gold nanoparticle film electrodes and multienzyme-particle amplification