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Reaction mixture II for each sample was collected as follows: 1160 l of 0.1-M Tris-HCl buffer with 0.34-mM EDTA (pH 8.5) were sequentially added to 108 l of each supernatant containing residual GSH, and then at the same intervals, 9 l of a solution of 5,5-dithiobis(2-nitrobenzoic acid) or Ellmans reagent (4 mg/ml of absolute methanol) was added to each sample (in the same sequence of samples)

doi: 10.1172/JCI69600 48 BoroughsLKDeBerardinisRJ

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In young and aged rats, treadmill exercise for a duration of 6 weeks led to increased SIRT1 activity, NAMPT and NAD + levels, and decreased mitochondrial generation of ROS 302