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In this study, an siRNA library of deubiquitylating enzymes (DUBs), expressed in HEK293 cells, was used to perform the screen in U87, U251, T98G, and LN229 GBM cell lines

Xin Z, Xiang Y, Wang Q, Bai X, Meng D, Wu J, et al

In practice, stacking multiplies uncertainty: more compounds more chances of impurity/contamination more pathways affected more interaction risk more variables harder to know what helped (or harmed) If you take only one clinical principle from this article: the more experimental the stack, the more conservative your risk posture should be

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