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Human astrocytes inhibit HIV-1 expression in monocyte-derived macrophages by secreted factors

To address these limitations, future research should prioritize: (1) developing more physiologically relevant preclinical models (e.g., humanized liver chimeras, diet-induced murine models with metabolic comorbidities) to better recapitulate human NAFLD pathophysiology

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Artificial optimized 5 untranslated regions (5 UTRs) including the ribosome binding sites (RBS) were generated for each gene and vector combination with the online RBS calculator of the Salis Laboratory of Penn State University 2 and were introduced via the oligonucleotides during cloning