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At the end of the experimental period, animals were exposed to the respective paradigms

Epigenetic modifications, particularly histone deacetylase (HDAC)-mediated chromatin remodeling, have emerged as a critical interface between chronic ethanol exposure and depressive phenotypes

Cells were stained with 0.2 M MitoSOX in prewarmed hanks balanced salt solution (HBSS), incubated in the dark at 37C and immediately analyzed using a FACS Canto II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA)

Consider implementing the following changes: Nutrition: Consuming whole foods through a balanced diet helps maintain hormonal equilibrium

Exosomes of adipose tissue may also have beneficial effects on NAFLD