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Lentiviral transduction of mRFP-GFP-LC3 shRNA and autophagic flux determination Lentiviruses encoding short hairpin RNAs (shRNA) to human mRFP-GFP-LC3-puro were constructed by Hanbio Biotechnology (Hanbio, Shanghai, China)

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As abdominal compartment syndrome leads to organ failure at an intra-abdominal pressure of 20 mmHg (Hunter and Damani, 2004