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Subramaniam MD, Kumar BA, Iyer M, Vellingiri B

Free Radic Biol Med 53(6):13461357

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Thus, a direct side-by-side comparison of RT-PCR and PCR targeting the 16S rRNA and the 16S rRNA gene, respectively, demonstrated that RT-PCR was able to provide up to a two-logarithm higher sensitivity of bacteria detection in comparison with the PCR-based assay [90, 100] and the sensitivity provided by RT-PCR is approaching the sensitivity of conventional microbiological culture methods [100]