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Glutamate cysteine ligase (GCL) catalyzes ligation of cysteine and glutamate forming -glutamylcysteine (-GC)

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Then, the cleavage reaction was dialyzed against 50 mM sodium phosphate (pH 7.4)

Indeed, GSH, Trx, Grx, Prx (together with the enzymes catalyzing their reduction), and the enzymatic antioxidant defense, as a whole, concur to modulate either ROS concentration, or the availability of cellular thiols, thus regulating the function of a large number of proteins implicated in the induction and/or execution of redox-sensitive pathways