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Finally, to confirm that the MyOne beads loaded with HS02-52G are able not only to capture APC from a purified system containing only APC, but also from the activation mixture containing generated APC, residual amounts of PC as well as human thrombin and hirudin, different amount of PC (5 g to 40 g Ceprotin) were subjected first to activation process (see section PC activation) and then to the optimized capture and elution process

Thus, the residual absorbance only represents unreacted substrates, providing a contamination-free result

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