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View source Current Pharmaceutical Design 2016 PMID 27138887 Sikiric P et al

[DOI] [PubMed] [Google Scholar] Claver JA, Quaglia AIE

There exists a complex interaction between the CNS and bone, with one key aspect being the neuroregulation of bone metabolism

Glutathione-sensitive linkers use higher concentrations of intracellular glutathione that in the plasma

The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation