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Bioavailability and pharmacokinetics of Dpenicillamine

After 48 h, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega), and firefly signals were normalized to Renilla signals to determine relative promoter activity

P., Cook, J

If you want a deeper dive into timing and format, How to Take Vitamin C Correctly is a helpful companion read

Mass-spectrometric analyses For absolute quantification of ubiquitin linkages (Ub-AQUA/PRM), analytes were separated using short-run SDS-PAGE and the gel pieces were washed in 50 mM AMBC/30% acetonitrile (ACN) for 2 h, in 50 mM AMBC/50% ACN for 1 h, and dehydrated in 100% ACN for 15 min