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Acute disruption of mitogen-activated protein kinase (MAPK) signaling and/or the blockage of PIK3 pathway paradoxically promotes tumor cell survival via anti-apoptotic mechanisms

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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

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