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However, for patients identified preoperatively with severe malnutrition or a high inflammatory state, a more aggressive nutritional strategy may be required, such as early combination with parenteral nutrition (PN) to ensure adequate energy and protein supply, or more cautious selection of feeding tube routes to balance the benefits of enteral nutrition with the risk of aspiration (120)

Cells were cultured and treated in a 37 C humidified incubator gassed with 5% CO 2 and 90% N 2 so as to achieve bone marrow level hypoxia of 5% O 2 or alternatively room air without N 2 to achieve 20% O 2

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Notably, this only partially overlaps with the distribution of chemical marker of hypoxia pimonidazole, HIF and other endogenous biomarkers of hypoxia (VEGF, GLUT-1, MCT-4) due to differences in hypoxic thresholds for induction, post-translational stabilities and additional regulatory factors at all expression levels [85]

J Biol Chem 2008;283:31203129