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The p150 protein was purified using the protocol described previously 44

If it does not dissolve after 5 minutes of gentle swirling, possible causes include: degraded peptide (check storage history and expiration status ), contaminated water (use a fresh vial of bacteriostatic water), or water temperature (cold water from the refrigerator dissolves peptides more slowly than room temperature water)

El factor intrnseco (FI) es una glicoprotena secretada por las clulas parietales de la mucosa gstrica que tiene la funcin de unirse a la vitamina B12 una vez sta se ha desprendido de las protenas R, otorgndole resistencia a las proteasas gstricas y pancreticas, formando un complejo B12+factor intrnseco que se conoce como complejo de Castle

The clinic answer In a UK clinic, cold-chain storage, stock rotation and expiry checks are routine, so you never have to think about any of this

Notably, it is difficult to calculate an accurate FDR using the entrapment strategy given that it is unlikely to know the exact number of phosphopeptides in the human sub-library that is not present in the sample