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Automated high-performance liquid chromatographic method for the determination of guanidinoacetic acid in dried blood spots: a tool for early diagnosis of guanidinoacetate methyltransferase deficiency

NS medium was prepared from 70 g 0.1 g L -1 seed extract supplemented with 0.1 g L -1 glucose, 20 g L -1 Bacto Agar, and 0.05% Tween 20

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The concentration of peptides in each sample was calculated referencing the standard curve

Beyond therapeutic innovation, however, the congress highlighted a broader transformation in healthcare: the growing importance of patient-centered care, the implementation of the new European Union Health Technology Assessment (HTA) Regulation, and the increasing role of Real-World Data (RWD) and Real-World Evidence (RWE) in bridging the gap between clinical trial results and outcomes achieved in everyday clinical practice