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Near infrared secondary antibody and CellTag700 total protein stain were added at desired concentration (Table S1) to 1.5% BSA and incubated with fixed cell culture overnight at 4 C

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In vitro studies using human retinal pigment epithelial (ARPE-19) cells exposed to oxidative (HO) and hyperglycaemic stress demonstrated that the probucolLCA nanoparticles significantly improved cell viability, reduced reactive oxygen species (ROS), restored glutathione (GSH) levels, and enhanced mitochondrial bioenergetics compared with non-functionalized formulations