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Immunocytochemistry Cells were fixed with 4% PFA (Thermo Fisher Scientific 28906) for 10 min at RT

The number of deaths is closely connected to the growth of innate immune cells in the lungs, mainly macrophages, which generate inflammatory cytokines (especially IL-6 and IL-1) that induce cytokine storm syndrome (CSS), multi-organ failure, and death

Blocking buffer was decanted and wells were washed thrice with PBS-T

Extracellular oligomeric A further exacerbates this instability by activating membrane receptors and forming de novo Ca2+-permeable pores in the plasma membrane (52)

Mitochondrial stress, induced by the application of electron transport chain inhibitors, triggers the activation of AMPK, which in turn phosphorylates FNIP1