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Lentivirus production Lentivirus was produced in HEK-293T cells which were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) with the plasmid of interest, a packaging plasmid psPAX2, a gift from Didier Trono (Addgene plasmid # 12260

Conclusion: Results demonstrate a low prevalence (2.4%) of CT head abnormality in cirrhotic patients presenting with hepatic encephalopathy and a low yield of routine CT head use in the absence of trauma or focal neurological deficit

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