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On the next day, the excess of the secondary antibody was removed by 2 washes in 0.1% Tween-20 in PBS, first rapid, second for 1 h at 4 C on an orbital shaker

The curious findings refer also to the sera of breast cancer women

Expression of an estrogen-regulated variant transcript of the peroxisomal branched chain fatty acid oxidase ACOX2 in breast carcinomas

The DEGs with known function that are positively regulated by Cyss deprivation in an NRF2-dependent manner are stress response genes involved in endoplasmic reticulum (ER) stress response and autophagy ( DDIT4 , CREB3L4 , DDIT3 , XAF1 , NUPR1, and GADD45B ) [32,33,34,35,36], mitophagy ( MORN4 ) [37] or mitochondrial biogenesis ( TFB1M ) [38]

also demonstrated this difference with the mutated CTNNB1 group showing significantly higher -catenin mRNA expression compared with the wild-type group (MannWhitney U test, p = 0.0036) [31]