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Cells were lysed in lysis solution (Pioneer, Shanghai, China) supplemented with protease inhibitors and phosphatase inhibitors (Target Mol, USA)

Acne, a prevalent skin condition, impacts millions globally, especially during adolescence and early adulthood

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In addition, CCL2 and CCL5 are considered to be the two main chemokines of TAMs, which responsible for attracting TAMs infiltration and inducing its polarization towards the oncogenic M2 phenotype whereas blocking CCL2 and CCL5 can block the occurrence of this phenomenon (52, 53), such as the mRNA encoding BisCCL2/5i encapsulated in a lipid nanoparticle platform can block both CCL2 and CCL5 signaling thereby significantly inducing TAMs to the anti-tumor M1 phenotype polarization and reversing immunosuppression in the TME (54)