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Description

Mapping non-PAT mediated GSTP1 palmitoylation sites by LC-MS/MS GSTP1 (2 g) was incubated with palmitoyl-CoA (100 M) or CoA (100 M) for 20 min at room temperature, and then digested for 6 h with 0.6 g Asp-N (which cuts on the NH 2 -terminal side of Asp and Cys residues) in 0.05% surfactant RapiGest SF at 37C