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Murphy DE, de Jong OG, Brouwer M, Wood MJ, Lavieu G, Schiffelers RM, Vader P

The external adverse elements, such as cigarette smoking, physical damage, and microbiota influence, may cause keratinocytes damaged through excessive production of ROS(Plenkowska et al., 2020)

doi: 10.1016/j.pain.2007.11.009 180 OcanaM.CendanC

In addition a 100 L of the blood was centrifuged at 10,000XGs for 10 min to isolate the serum and the lactate and NAD + /NADH ratio in the plasma were determined using lactate (Abcam Inc.) and NADH enzymatic kits (Sigma Aldrich) (figure 3B, D)