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Immunofluorescence, confocal microscopy image acquisition and colocalization analysis For the immunofluorescence analysis of endogenous proteins, RAW 264.7 murine macrophages (ATCC, TIB-71) were seeded on untreated coverglass using a four-chamber 35-mm dish with 20-mm microwells (Cellvis, D35C4-20-1.5-N), incubated at 37 C for 30 min, then fixed with 3.7% paraformaldehyde for 8 min, permeabilized with 0.01% saponin and blocked with 1% BSA in PBS