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In Chlamydomonas reinhardtii , genetic disruption of TTLL9 demonstrated that long-chain -tubulin polyglutamylation is dispensable for axonemal assembly but essential for proper flagellar motility [53]

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The cell pellet was homogenized in NP40 lysis buffer (1% NP40, 150 mM NaCl, 50 mM Tris-Cl, pH 8.0) on ice for 30 min and centrifuged for 5 min at 20,000 g 4C