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For example, the interaction between ApoE and TREM2, another major risk factor for sporadic AD, has been reported to facilitate the uptake of A by microglia, and AD-linked mutations in TREM2 impair the binding affinity of TREM2 for ApoE leading to A accumulation in the brain (Yeh et al., 2016)

Serial applications of oligomycin (ATP synthase inhibitor, 1 M), FCCP (protonophore, 0.5 M), and rotenone and antimycin A (respiratory complex I and III inhibitor, respectively, 0.5 M) over time enabled calculation of various parameters of mitochondrial respiration in both cell lines

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