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SAS is able to inhibit SystemX c thereby inducing ferroptosis in addition to inhibition of NF-B pathway (Gout et al., 2001)

The excessive ROS production during reperfusion can lead to the opening of the mitochondrial permeability transition pore (mPTP), causing mitochondrial swelling, loss of membrane potential, and cell death [2]

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Once inside the cell, the sulfhydryl (-SH) group of the glutathione molecule acts as a potent reducing agent, directly donating an electron to unstable reactive oxygen species

Caspase-3 activity assay Both hepatocytes and liver tissues were lysed on ice with lysis buffer containing protease inhibitors, and the proteins were collected