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For purification, the cell pellet was resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM DTT, and 5% Glycerol) and sonicated to lyse the cells

10.1016/j.tips.2016.05.012 Trends Pharmacol

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After centrifugation, liver protein supernatant was incubated with reaction mixture (0.1 mol/L serine, 5 mmol/L EDTA, and 2.5 mmol/L propargylglycine in Tris buffer (1 mol/L, pH 8.4)), 2.5 mmol/L pyridoxalphosphate and homocysteine reagent for 1 h at 37C