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El-Rhermoul FZ, Fedorowski A, Eardley P, Taraborrelli P, Panagopoulos D, Sutton R, et al

The first-round PCR was conducted in a 32 l reaction containing 1.6 l of cell lysate, 1.6 l each of 10 M forward primer KI#168 and 10 M reverse primer KI#169, 0.64 l of Phusion High-Fidelity DNA Polymerase (NEB, no