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86 Novel Medicines and Future Perspectives of COPD Therapies Searching for an effective treatment for COPD is still ongoing and new drugs are under development

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Following 3 hours of feeding the CR mice at the start of the light cycle, all dietary groups underwent a 3 hr fast, followed by cervical dislocation and tissues for molecular analysis were flash-frozen in liquid nitrogen or fixed and prepared as described in the methods below

doi: 10.1007/s00125-019-4909-y 5 ChoNHShawJEKarurangaSHuangYda Rocha FernandesJDOhlroggeAWet al

Clinical evaluation Participants underwent a thorough psychiatric evaluation by trained psychologists using the K-SADS [53] during the baseline evaluation (V0)