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Using multiple high-throughput proteomics assays in two independent cohorts of konzo-affected children compared to unrelated individuals (Discovery) or their unaffected sibling (Validation) respectively, we demonstrate that levels of the critical plasma-based antioxidant enzyme Glutathione Peroxidase 3 (GPx3) are significantly reduced in those affected

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To explore if these large capsid aggregates were C-capsids, we compared the VP5 expression pattern formed by wild-type virus with those generated by the viral mutant vFH421 coding for HSV-1 1-50 U L 25, which produces highly reduced C-capsid levels but still efficiently makes other capsid types 34,36,82,83

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