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The Z-track method is recommended to administer IM injections as a way to reduce local tissue irritation (Astle & Duggleby, 2019)

Iino C, Shimoyama T, Iino K, et al

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For the LEfSe analysis, an alpha of 0.05 was set for the KruskalWallis tests among classes, and per-sample normalization was performed to bring the sum of the values to 1 M