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To prepare it for laboratory use, it must be reconstituted with a sterile diluent, most commonly Bacteriostatic Water

It is particularly effective for separating polar, non-volatile compounds such as Vitamin B3 in complex sample matrices

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These overwhelm standard growth factors, as all are rapidly destroyed in human gastric juice within 15 min [72], are unable to be applied alone, and require the addition of various carriers or biological scaffolds or various combinations (for review, see [14])