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Cells were re-suspended in 200 ml of lysis buffer, consisting of 350 mM NaCl, 10 mM Tris (pH 7.4), 1:10,000 -mercaptoethanol and supplemented with 100 l 4-benzene sulfonyl fluoride hydrochloride (AEBSF), MnCl 2 and deoxyribonuclease I

(Reference Skarpanska-Stejnborn, Pilaczynska-Szczesniak and Basta27) , which the authors interpreted as an indication that RR led to more effective elimination of superoxide anions in erythrocytes

Anti-inflammatory effects NAD + is essential for the activity of sirtuins, a class of proteins with anti-inflammatory properties

Keep tightly capped and stored in a cool, dry place for optimal freshness

Once mixed solution must remain cold at all times