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After a series of post-hybridization washing and blocking, Flu-positive cells were visualized with anti-FITC antibody (PerkinElmer, #NEF710001EA, 1:250 in blocking buffer) followed by TSA biotin amplification reagent (PerkinElmer, #NEF749A001KT, 1:50 in 1 plus amplification diluent) and streptavidin Alexa488 (Invitrogen, #S11223, 1:250 in blocking buffer)

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Microphthalmia associated transcription factor (MITF) is a transcription factor important for melanogenesis genes (Hearing 1999)

These subtypes can change progressively and become more resistant to chemoradiotherapy (214)

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