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Inside the cell, it is enzymatically cleaved by cytoplasmic esterases to form DAF-FM, which remains intracellular since it cannot cross the membrane

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531 More accurate studies of morphology of astrocytes in aged mice in situ using intracellular injections of fluorescent dye Alexa Fluor 594 with subsequent two-photon imaging and 3D reconstruction identified a substantial reduction of astrocytic size and complexity with particular degradation of perisynaptic leaflets (Fig

172 Wnt/-catenin pathway in liver disease The liver is a metabolic factory in humans