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Then, cells were resuspended in 300 l hybridization mixture (formamide 70%, blocking reagent 1, Tris-HCl, pH 7.4, 10 mM, locked nucleic acid probe 0.5 mM)

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Although a significantly elevated renal CAT activity in 6- and 16-week old, 22 and older SHR 8, 23 has been reported before, this is the first time that altered renal CAT expression and activity has been reported in pre-hypertensive SHR