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Typically, a dendritic spine consists of three basic compartments: (1) a delta-shaped base which contains branched actin filaments residing on the microtubule network in the dendrites, (2) a constricted neck in the middle which contains branched and linear longitudinal actin filaments, and (3) a bulbous head contacting the axon, which usually contains a dense network of short cross-linked branched actin filament (Hotulainen and Hoogenraad, 2010)

The reaction was held at 180 C under nitrogen for 10 min, and then cooled to room temperature and precipitated with isopropanol and re-dispersed in methylcyclohexane