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To downregulate TfRs and limit the amount of surface binding or endocytosis in HL-60 cells, 100 M human holo-Tf (Sigma-Aldrich) in endotoxin-free PBS (EMD Millipore) was added to the medium for overnight incubation

Siva et al., 2022)

Rinse and squeeze out excess water after each use

Glutathione also helps in retaining the collagen present in the skin, this becomes vital in maintaining the firmness and elasticity of the skin

To establish stable knockdown cell lines targeting STK38, STK38L, RIPK3, and mStk38, HEK293T cells were transfected with packaging plasmids and the following pLKO.1-puro vectors: shGFP, shSTK38 #1, shSTK38 #3, shSTK38L, shRIPK3, shmStk38 #1, shmStk38 #2, to generate lentiviral particles