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By contrast, the protein purity by IMAC is relatively crude, a time-consuming second purification step, e.g., ion exchange chromatography is essential for sensitive applications

40 NagarS.WaltherS.BlanchardR

Functionalization of 3-PLG with l-alanine and l-glutathione The A-GSH-targeted 3-PLGs (3-PLG-A-GSH) were synthesized by grafting A and GSH on 3-PLG using N -ethyl- N -(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling reaction in DIW (Fig