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Plant Mol Biol 69:577592 Edwards EA, Rawsthorne S, Mullineaux PM (1990) Subcellular distribution of multiple forms of glutathione reductase in leaves of pea ( Pisum sativum L.)

However, in cancer cells, mutations in KEAP1 or NRF2 hyperactivate NRF2, protecting them from oxidative stress and therapy-induced damage [19]

To measure the CRT expressions, after treatment, the cells were washed with cold PBS, and further incubated with anti-CRT polyclonal antibody (diluted 1:100, anti-human antibody for G7, WL1, U87, and U251 cells and anti-mouse antibody for GL261 and G422 cells) at 4 C for 30 min, and stained with Alexa Fluor 647 labeled goat antirabbit IgG (H&L), a highly cross-adsorbed secondary antibody (1:200 dilution), for 30 min at room temperature

The test was repeated thrice in a row, and the mean value of the holding force was considered

doi: 10.1124/jpet.108.149815 110 ZhangYGLuRWuSChatterjeeIZhouDXiaYet al