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Parkin, a tumor suppressor, ubiquitinates PHGDH at K330, with its IBR domain binding to PHGDH-SBD2

Wang X, Lu Q, Guo J, Ares I, Martinez M, Martinez-Larranaga MR, et al

I did feel dizzy slightly but Ive had nerve issues in the past

Subsequently, the cells were co-incubated overnight with an anti-cytochrome c antibody, followed by a 2-h incubation with an Alexa Fluor 488 conjugated secondary antibody