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In this study, iPSCs have been differentiated using DMEM/F12 medium supplemented with L-glutamin, heparin, and B27 for astrocytes or vasculife basal medium supplemented with FGF, acid ascorbic, hydrocortisone, glutamine, and growth factor for brain endothelial cells

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doi:10.1155/2021/6564585 [Made by author on BioRender] The interactions of these different cell types together with efficient junctions and the multilayer structure ensure protection of the brain from harmful substances