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Instrumental conditions UV spectroscopy A Genesys 10S UV-VIS spectrophotometer (Thermo Fischer Scientific, Madison, USA) was used for the absorption measurements at 361 nm

Total protein concentration in isolated synaptosomal fractions was estimated using Pierce BCA protein assay reagents (Pittsburgh PA, USA)

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( E ) Representative Western blots show decreased expression of GPX4 under conditions of 15LO1 inhibition ( n = 4)

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