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Following another wash with PBS, sections were incubated with primary antibodies recognising: activated resident microglia/macrophages (IBA1, 2 g/ml, 1:500, Abcam, goat Ab5076)

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In vitro study using human vascular endothelial cells further confirmed the increased mRNA and protein expressions of VEGFR2 but not VEGF-A

Written informed consent was obtained from bladder cancer patients