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To determine the dual-color STED point-to-point resolution, GATTA Dual color 70 nm Alexa 647/ Alexa-594/ Alexa 647 spaced beads were imaged in confocal and STED with 775 nm depletion laser with 0% Z -depletion (Supplementary Figure 2g, h)

Time-to-complications (an endpoint of either ascites, variceal bleeding, encephalopathy, liver transplant or death) from enrollment into deep or adequate response cohorts was compared between deep and adequate response groups via a Cox proportional hazard model