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Furthermore, high ROS levels trigger the apoptotic cascade by cleaving caspase 8 (CAS-8) and subsequently activating caspase 3 (CAS-3), which in turn degrades nucleic acids 40

Carotenoids, particularly -carotenoids, have free radical scavenging characteristics, quenching highly reactive singlet molecular oxygen ( 1 O 2 ) and reducing oxidative damage to cells

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Then, the hydrogels were immersed in PBS (pH 5.5, pH 6.5 or pH 7.4) and incubated at 37 C with gentle shaking