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Iron dextran treatment was stopped after three weeks, and deferoxamine mesylate and VITE intervention were extended for another week

Mice were fed customized control chow diet containing 0.5% bile salt and 10% fat, or high cholesterol diet (CH diet, chow diet supplemented with 0.1% purified cholesterol) (Trophic, Nanjing, China, TP23302)

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Hypotaurine, a metabolite derived from cysteine sulfinic acid metabolism, was also increased in replicative senescent fibroblasts, with respect to control cells (James et al., 2016